{"id":1300097,"date":"2018-05-09T20:47:05","date_gmt":"2018-05-10T03:47:05","guid":{"rendered":"http:\/\/www.sandiegochinesepress.com\/press\/?p=1300097"},"modified":"2018-05-09T20:47:56","modified_gmt":"2018-05-10T03:47:56","slug":"%e8%8d%af%e6%98%8e%e5%ba%b7%e5%be%b7%ef%bc%9a%e7%bb%86%e8%83%9e%e5%ad%98%e6%b4%bb%e7%8e%87%e6%8f%90%e9%ab%987%e5%80%8d%ef%bc%81%e6%9b%b4%e7%b2%be%e5%87%86%e7%9a%84crispr%e8%af%9e%e7%94%9f%e4%ba%86","status":"publish","type":"post","link":"https:\/\/www.sandiegochinesepress.com\/press\/%e8%8d%af%e6%98%8e%e5%ba%b7%e5%be%b7%ef%bc%9a%e7%bb%86%e8%83%9e%e5%ad%98%e6%b4%bb%e7%8e%87%e6%8f%90%e9%ab%987%e5%80%8d%ef%bc%81%e6%9b%b4%e7%b2%be%e5%87%86%e7%9a%84crispr%e8%af%9e%e7%94%9f%e4%ba%86\/.html","title":{"rendered":"\u836f\u660e\u5eb7\u5fb7\uff1a\u7ec6\u80de\u5b58\u6d3b\u7387\u63d0\u9ad87\u500d\uff01\u66f4\u7cbe\u51c6\u7684CRISPR\u8bde\u751f\u4e86 \uff01"},"content":{"rendered":"

\u836f\u660e\u5eb7\u5fb7\/\u62a5\u9053<\/strong><\/p>\n

\u4f7f\u7528CRISPR\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u5bf9DNA\u8fdb\u884c\u7f16\u8f91\u65f6\u5e38\u88ab\u55bb\u4e3a\u7528\u526a\u5200\u5bf9\u65b0\u95fb\u7a3f\u8fdb\u884c\u526a\u8d34\u3002\u7136\u800c\uff0c\u526a\u8d34\u8bcd\u53e5\u76f8\u5bf9\u5bb9\u6613\uff0c\u5220\u9664\u5355\u4e2a\u5b57\u7b26\u6216\u8005\u7acb\u5373\u4e86\u89e3\u526a\u8d34\u5bf9\u6587\u5b57\u610f\u601d\u7684\u5f71\u54cd\u5374\u5341\u5206\u56f0\u96be\u3002\u79d1\u5b66\u5bb6\u4eec\u5e0c\u671b\u80fd\u591f\u8fd0\u7528CRISPR\u6280\u672f\u5c06DNA\u4e2d\u81f4\u75c5\u7684\u57fa\u56e0\u53d8\u5f02\u5207\u9664\uff0c\u5e76\u66ff\u6362\u6210\u6b63\u5e38\u7684DNA\u5e8f\u5217\u3002\u4e3a\u4e86\u8fbe\u6210\u8fd9\u4e00\u76ee\u6807\uff0cCRISPR\u6280\u672f\u7684\u7cbe\u786e\u6027\u4ecd\u7136\u9700\u8981\u5f97\u5230\u63d0\u9ad8<\/strong>\u3002<\/p>\n

<\/p>\n

\u25b2<\/strong>CRISPR\u6280\u672f\u5e38\u88ab\u55bb\u4e3a\u201c\u57fa\u56e0\u526a\u5200\u201d\uff08\u56fe\u7247\u6765\u6e90\uff1a123RF.com\uff09<\/strong><\/p>\n

\u65e5\u524d\uff0c\u7531\u65af\u5766\u798f\u5927\u5b66(Stanford University)\u548c\u7f8e\u56fd\u56fd\u5bb6\u6807\u51c6\u4e0e\u6280\u672f\u7814\u7a76\u9662(NIST)\u8054\u5408\u6784\u5efa\u7684\u751f\u7269\u8ba1\u91cf\u5b66\u8054\u5408\u5021\u8bae(JIMB)\u7ec4\u7ec7\u7684\u7814\u7a76\u4eba\u5458\u5f00\u53d1\u51fa\u4e00\u79cd\u540d\u4e3aMAGESTIC<\/strong>\u7684\u4e0b\u4e00\u4ee3CRISPR\u6280\u672f\u5e73\u53f0\u3002\u8fd9\u4e00\u5e73\u53f0\u5c06CRISPR\u6280\u672f\u4ece\u7c97\u7cd9\u7684\u526a\u8d34\u5de5\u5177\uff0c\u53d8\u4e3a\u9ad8\u7ea7\u7684\u6587\u5b57\u5904\u7406\u5668\uff0c\u5229\u7528\u7c7b\u4f3c\u201c\u67e5\u8be2\u201d\u4e0e\u201c\u66ff\u6362\u201d\u7684\u529f\u80fd\u5bf9\u9057\u4f20\u7269\u8d28\u8fdb\u884c\u7cbe\u786e\u7f16\u8f91<\/strong>\u3002\u8fd9\u4e00\u6210\u679c\u53d1\u8868\u5728\u300aNature Biotechnology\u300b\u4e0a\u3002<\/p>\n

\u4f7f\u7528CRISPR\u6280\u672f\u5bf9\u57fa\u56e0\u7ec4\u8fdb\u884c\u7f16\u8f91\u9700\u8981\u7cbe\u786e\u5b8c\u6210\u4ee5\u4e0b\u51e0\u4e2a\u6b65\u9aa4\uff1a1.\u5728\u7ec6\u80deDNA\u7684\u7279\u5b9a\u4f4d\u70b9\u8fdb\u884c\u526a\u5207\uff1b2.\u5728\u5207\u5f00\u7684\u4f4d\u70b9\u51c6\u786e\u5f15\u5165\u9700\u8981\u52a0\u5165\u57fa\u56e0\u7ec4\u7684\u5e8f\u5217\uff1b3.\u51c6\u786e\u89c2\u5bdf\u5230\u57fa\u56e0\u7f16\u8f91\u4ea7\u751f\u7684\u540e\u679c\u3002\u76ee\u524d\u7684CRISPR\u6280\u672f\u4f7f\u7528\u6307\u5bfcRNA(guide RNA\uff0cgRNA)\u5f15\u5bfc\u5bf9DNA\u5e8f\u5217\u7684\u7cbe\u786e\u526a\u5207\uff0c\u4f9b\u4f53DNA(donor DNA)\u5e2e\u52a9\u5c06\u9700\u8981\u52a0\u5165\u7279\u5b9a\u4f4d\u70b9\u7684DNA\u5e8f\u5217\u5f15\u5165\u57fa\u56e0\u7ec4\u3002<\/p>\n

\u76ee\u524d\uff0cgRNA\u80fd\u591f\u5f15\u5bfc\u5bf9\u57fa\u56e0\u7ec4\u4e2d\u7279\u5b9a\u4f4d\u70b9\u7684\u7cbe\u786e\u526a\u5207\uff0c\u4f46\u662f\u4e0b\u4e00\u6b65\u7684DNA\u4fee\u8865\u8fc7\u7a0b\u5f80\u5f80\u4e0d\u591f\u7cbe\u786e\uff0c\u5f88\u591a\u65f6\u5019\u4fee\u8865\u8fc7\u7a0b\u4f1a\u5f15\u5165\u968f\u673a\u7a81\u53d8\uff0c\u800c\u4eba\u5de5\u8868\u8fbe\u7684\u4f9b\u4f53DNA\u5e8f\u5217\u4e0d\u80fd\u6709\u6548\u5730\u6574\u5408\u5230\u57fa\u56e0\u7ec4\u4e2d\uff0c\u8fd9\u5bfc\u81f4\u5927\u91cf\u7ec6\u80de\u5728\u57fa\u56e0\u7f16\u8f91\u8fc7\u7a0b\u4e2d\u6b7b\u4ea1<\/strong>\u3002\u5bf9\u4e8e\u7ec6\u80de\u6765\u8bf4\uff0c\u8ba9DNA\u4fee\u590d\u673a\u5236\u5728\u5305\u542b\u4e0a\u767e\u4e07\u5230\u4e0a\u4ebf\u4e2a\u78b1\u57fa\u5bf9\u7684DNA\u5e8f\u5217\u4e2d\u627e\u5230\u5408\u9002\u7684\u4f9b\u4f53DNA\u662f\u4e00\u4ef6\u5f88\u6709\u6311\u6218\u6027\u7684\u5de5\u4f5c\u3002MAGESTIC\u5e73\u53f0\u5728\u8fd9\u65b9\u9762\u7684\u8fdb\u6b65\u662f\u4f7f\u7528\u4e86\u540d\u4e3a\u201c\u4e3b\u52a8\u4f9b\u4f53\u52df\u96c6\uff08active donor recruitment\uff09<\/strong>\u201d\u7684\u624b\u6bb5\uff0c\u5c06\u4eba\u5de5\u5408\u6210\u7684\u4f9b\u4f53DNA\u7247\u6bb5\u52df\u96c6\u5230\u526a\u5207\u4f4d\u70b9\u7684\u5468\u56f4\u3002\u8fd9\u4e00\u52df\u96c6\u624b\u6bb5\u8ba9\u7ec6\u80de\u5b58\u6d3b\u7387\u63d0\u9ad8\u4e86\u4e03\u500d<\/strong>\uff01<\/p>\n

<\/p>\n

\u25b2MAGESTIC\u5e73\u53f0\u4ee5\u65b0\u7684\u65b9\u5f0f\u4f7f\u7528\u7ec6\u80de\u6761\u5f62\u7801\uff0c\u4e3aCRISPR\u57fa\u56e0\u7f16\u8f91\u589e\u52a0\u4e86\u65b0\u7684\u7cbe\u5ea6\u7ea7\u522b\uff08\u56fe\u7247\u6765\u6e90\uff1aKelly Irvine\/NIST\uff09<\/strong><\/p>\n

MAGESTIC\u4e0e\u4ee5\u5f80\u9ad8\u901a\u91cfCRISPR\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u7684\u53e6\u4e00\u4e2a\u663e\u8457\u533a\u522b\u5728\u4e8e\uff0c\u7ec6\u80de\u6761\u5f62\u7801\u7684\u8bbe\u8ba1<\/strong>\u3002\u8fc7\u53bb\uff0c\u7814\u7a76\u4eba\u5458\u901a\u5e38\u7528\u8d28\u7c92(plasmid)\u6765\u8868\u8fbegRNA\uff0c\u5e76\u5b58\u50a8\u6761\u5f62\u7801\u5e8f\u5217\u4ee5\u8ffd\u8e2a\u7ec6\u80de\u7684\u7a81\u53d8\uff0c\u8fd9\u4e9b\u8d28\u7c92DNA\u4f1a\u968f\u7740\u7ec6\u80de\u7684\u751f\u957f\u548c\u5206\u88c2\u8fdb\u884c\u590d\u5236\uff0c\u5e76\u4f20\u9012\u5230\u4e0b\u4e00\u4ee3\u7ec6\u80de\u4e2d\u3002\u4f46\u662f\uff0c\u6bcf\u4e2a\u7ec6\u80de\u4e2d\u8d28\u7c92DNA\u7684\u6570\u91cf\u53ef\u4ee5\u4ece10\u4e2a\u523040\u4e2a\u4e0d\u7b49\uff0c\u8fd9\u4f1a\u5bfc\u81f4\u6761\u5f62\u7801\u7684\u6570\u91cf\u65e0\u6cd5\u7528\u6765\u7cbe\u786e\u68c0\u6d4b\u7ec6\u80de\u7684\u6570\u76ee\u3002\u65b0\u7684MAGESTIC\u6280\u672f\u5e73\u53f0\u80fd\u5c06\u6761\u5f62\u7801\u6574\u5408\u5230\u7ec6\u80de\u7684\u67d3\u8272\u4f53\u4e2d\uff0c\u8fd9\u4e00\u6539\u8fdb\u8ba9\u6761\u5f62\u7801\u7684\u6570\u76ee\u53d8\u5f97\u7a33\u5b9a\uff0c\u4f7f\u5b83\u4eec\u53ef\u4ee5\u66f4\u7cbe\u786e\u5730\u9884\u6d4b\u7ec6\u80de\u7684\u6570\u91cf\u3002<\/p>\n

\u867d\u7136\u5728\u8fc7\u53bb20\u5e74\u91cc\uff0c\u5bf9DNA\u7684\u6d4b\u5e8f\u548c\u7f16\u8f91\u6280\u672f\u51fa\u73b0\u4e86\u5927\u5e45\u5ea6\u7684\u8fdb\u5c55\uff0c\u4f46\u662f\u79d1\u5b66\u5bb6\u4eec\u5bf9\u57fa\u56e0\u7ec4\u4e2d\u5b58\u5728\u7684\u81ea\u7136\u53d8\u5f02\u7684\u529f\u80fd\u548c\u5b83\u4eec\u5bf9\u75be\u75c5\u7684\u5f71\u54cd\u4ecd\u7136\u7f3a\u4e4f\u4e86\u89e3\u3002MAGESTIC\u53ef\u4ee5\u901a\u8fc7\u7cbe\u786e\u5730\u7f16\u8f91\u6bcf\u4e00\u4e2a\u81ea\u7136\u53d1\u751f\u7684\u57fa\u56e0\u53d8\u5f02\uff0c\u5e76\u4e14\u628a\u5b83\u4eec\u548c\u5176\u5b83\u57fa\u56e0\u53d8\u5f02\u8fdb\u884c\u6bd4\u8f83\u6765\u589e\u8fdb\u5bf9\u81ea\u7136\u53d8\u5f02\u7684\u7406\u89e3\u3002MAGESTIC\u7684\u53e6\u4e00\u4e2a\u4f18\u52bf\u662f\u80fd\u591f\u5728\u4e00\u4e2a\u8bd5\u7ba1\u91cc\u5bf9\u4e0a\u767e\u4e07\u4e2a\u7ec6\u80de\u8fdb\u884c\u9ad8\u901a\u91cf\u57fa\u56e0\u7f16\u8f91<\/strong>\uff0c\u800c\u8fc7\u53bb\u7684\u6280\u672f\u5e73\u53f0\u9700\u8981\u4e0d\u540c\u5b9e\u9a8c\u6765\u5bf9\u6bcf\u4e2a\u57fa\u56e0\u53d8\u5f02\u8fdb\u884c\u7f16\u8f91\u3002<\/p>\n

\u201c\u73b0\u6709\u7684\u6280\u672f\u8fdb\u6b65\u8ba9\u6211\u4eec\u4e0d\u4f46\u80fd\u591f\u5bf9\u57fa\u56e0\u7ec4\u7684\u6bcf\u4e2a\u78b1\u57fa\u5bf9\u8fdb\u884c\u6d4b\u5e8f\uff0c\u800c\u4e14\u80fd\u591f\u5bf9\u5b83\u4eec\u8fdb\u884c\u4fee\u6539\u3002\u4f46\u662f\u6211\u4eec\u4ecd\u7136\u9700\u8981\u66f4\u597d\u5730\u7406\u89e3\u4fee\u6539\u7684\u540e\u679c\uff0c\u201d\u8fd9\u7bc7\u8bba\u6587\u7684\u8d44\u6df1\u4f5c\u8005\u3001\u65af\u5766\u798f\u5927\u5b66\u9057\u4f20\u5b66\u6559\u6388Lars Steinmetz\u535a\u58eb\u8bf4\uff1a\u201cMAGESTIC\u5e73\u53f0\u597d\u6bd4\u8ba9\u6211\u4eec\u5728\u57fa\u56e0\u7ec4\u8fd9\u672c\u4e66\u4e2d\u5bf9\u5355\u4e2a\u5b57\u7b26\u8fdb\u884c\u4fee\u6539\uff0c\u7136\u540e\u89c2\u5bdf\u8fd9\u79cd\u4fee\u6539\u5bf9\u6587\u5b57\u610f\u601d\u7684\u5f71\u54cd\u3002\u800c\u6211\u4eec\u7684\u4f9b\u4f53\u52df\u96c6\u624b\u6bb5\u8ba9\u9700\u8981\u6dfb\u52a0\u7684\u65b0\u4fe1\u606f\u88ab\u7cbe\u786e\u653e\u7f6e\u5230\u526a\u5207\u51fa\u73b0\u7684\u90a3\u4e00\u9875\u4e2d\u3002\u201d<\/p>\n

\u53c2\u8003\u8d44\u6599\uff1a<\/p>\n

[1] Taking CRISPR from clipping scissors to word processor<\/p>\n

[2] Multiplexed precision genome editing with trackable genomic barcodes in yeast<\/p>\n

 <\/p>\n

<\/p>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

\u4f7f\u7528CRISPR\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u5bf9DNA\u8fdb\u884c\u7f16\u8f91\u65f6\u5e38\u88ab\u55bb\u4e3a\u7528\u526a\u5200\u5bf9\u65b0\u95fb\u7a3f\u8fdb\u884c\u526a\u8d34\u3002\u7136\u800c\uff0c\u526a\u8d34\u8bcd\u53e5\u76f8\u5bf9\u5bb9\u6613\uff0c\u5220\u9664\u5355\u4e2a\u5b57\u7b26\u6216\u8005\u7acb\u5373\u4e86\u89e3\u526a\u8d34\u5bf9\u6587\u5b57\u610f\u601d\u7684\u5f71\u54cd\u5374\u5341\u5206\u56f0\u96be\u3002\u79d1\u5b66\u5bb6\u4eec\u5e0c\u671b\u80fd\u591f\u8fd0\u7528CRISPR\u6280\u672f\u5c06DNA\u4e2d\u81f4\u75c5\u7684\u57fa\u56e0\u53d8\u5f02\u5207\u9664\u3002\u3002\u3002\u3002\u3002\u3002<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[16],"tags":[],"class_list":["post-1300097","post","type-post","status-publish","format-standard","hentry","category-science_technology_education"],"_links":{"self":[{"href":"https:\/\/www.sandiegochinesepress.com\/press\/wp-json\/wp\/v2\/posts\/1300097","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sandiegochinesepress.com\/press\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.sandiegochinesepress.com\/press\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.sandiegochinesepress.com\/press\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.sandiegochinesepress.com\/press\/wp-json\/wp\/v2\/comments?post=1300097"}],"version-history":[{"count":0,"href":"https:\/\/www.sandiegochinesepress.com\/press\/wp-json\/wp\/v2\/posts\/1300097\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.sandiegochinesepress.com\/press\/wp-json\/wp\/v2\/media?parent=1300097"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sandiegochinesepress.com\/press\/wp-json\/wp\/v2\/categories?post=1300097"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.sandiegochinesepress.com\/press\/wp-json\/wp\/v2\/tags?post=1300097"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}