UCSD\u751f\u7269\u7cfb\u6bd5\u4e1a\u7684\u65b9\u82b3\u535a\u58eb\u8bf4\uff1a\u201d \u6211\u4eec\u5728UCSD\u7684\u65f6\u5019\u5e38\u5e38\u5728\u7cfb\u91cc\u89c1\u5230\u4ed6\uff0c\u6ca1\u6709\u4e00\u70b9\u67b6\u5b50\uff0c\u5f00\u7814\u8ba8\u4f1a\u4ed6\u90fd\u4f1a\u6765\u53c2\u52a0\uff0c\u6709\u65f6\u5750\u5728\u6700\u540e\u4e00\u6392\uff0c\u4e0d\u8fc7\u4ed6\u53d1\u95ee\u5f88\u7280\u5229\uff0c\u597d\u591a\u5b66\u751f\u8bf7\u4ed6\u4f5c\u5b66\u672f\u8bc4\u59d4\u65f6\u90fd\u4e8b\u5148\u77e5\u9053\u8981\u597d\u597d\u5730\u505a\u597d\u51c6\u5907\uff0c\u4ed6\u4e00\u4e0b\u5c31\u80fd\u542c\u51fa\u95ee\u9898\u7684\u5173\u952e\u5728\u54ea\u91cc\u3002\u201c <\/span><\/p>\n\u4e8c\uff1a<\/p>\n
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\u4e09\uff1a<\/p>\n
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\u3000\u3000-\u9976\u6bc5<\/p>\n
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\u73b0\u5728\u7814\u7a76\u751f\u7269\u7684\u4eba\uff0c\u51e0\u4e4e\u90fd\u77e5\u9053\u7eff\u8272\u8367\u5149\u86cb\u767d\uff08GFP\uff09\uff0c\u4f46\u5e38\u5e38\u4e0d\u77e5\u6216\u641e\u9519\u5176\u53d1\u73b0\u8005\u3002\u6beb\u65e0\u4e89\u8bae\u7684\u53d1\u73b0\u8005\u662f\u65e5\u88d4\u7f8e\u56fd\u79d1\u5b66\u5bb6\u4e0b\u6751\u4fee\uff08Osamu Shimomura\uff0c\u4e0b\u6751\u8129\uff09\u548c\u5df2\u6545\u7f8e\u56fd\u79d1\u5b66\u5bb6\u7ea6\u7ff0\u68ee\uff08Frank H. Johnson\uff09\u3002\u4ed6\u4eec1961\u52301974\u5e74\u53d1\u73b0\u4e24\u79cd\u53d1\u5149\u7684\u86cb\u767d\u8d28\uff1a\u6c34\u6bcd\u7d20\uff08aequorin\uff09\u548cGFP\u3002<\/p>\n
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\u4e0b\u6751\u4fee\u548c\u7ea6\u7ff0\u68ee\u7528\u8fc7\u51e0\u79cd\u5b9e\u9a8c\u52a8\u7269\uff0c\u548c\u672c\u6545\u4e8b\u76f8\u5173\u7684\u662f\u5b66\u540d\u4e3aAequorea victoria\u7684 \u6c34\u6bcd\u30021962\u5e74\uff0c\u4e0b\u6751\u4fee\u548c\u7ea6\u7ff0\u68ee\u7b49\u5728\u300a\u7ec6\u80de\u548c\u6bd4\u8f83\u751f\u7406\u5b66\u6742\u5fd7\u300b\u4e0a\u62a5\u9053\uff0c\u4ed6\u4eec\u5206\u79bb\u7eaf\u5316\u4e86\u6c34\u6bcd\u4e2d\u53d1\u5149\u86cb\u767d\u6c34\u6bcd\u7d20\u3002\u636e\u8bf4\u4e0b\u6751\u4fee\u7528\u6c34\u6bcd\u63d0\u53d6\u53d1\u5149\u86cb\u767d\u65f6\uff0c\u6709\u5929\u4e0b\u73ed \u8981\u56de\u5bb6\u4e86\uff0c\u4ed6\u628a\u4ea7\u7269\u5012\u8fdb\u6c34\u6c60\u91cc\uff0c\u4e34\u51fa\u95e8\u524d\u5173\u706f\u540e\uff0c\u56de\u5934\u770b\u4e00\u773c\u6c34\u6c60\uff0c\u7ed3\u679c\u89c1\u6c34\u6c60\u95ea\u95ea\u53d1\u5149\u3002\u56e0\u4e3a\u517b\u9c7c\u7f38\u7684\u6c34\u4e5f\u6d41\u5230\u540c\u4e00\u6c34\u6c60\uff0c\u4ed6\u6000\u7591\u662f\u9c7c\u7f38\u6392\u51fa\u7684\u6210\u5206\u5f71\u54cd\u6c34\u6bcd \u7d20\uff0c\u4e0d\u4e45\u4ed6\u5c31\u786e\u5b9a\u9499\u79bb\u5b50\u589e\u5f3a\u6c34\u6bcd\u7d20\u53d1\u5149\u30021963\u5e74\uff0c\u4ed6\u4eec\u5728\u300a\u79d1\u5b66\u300b\u6742\u5fd7\u62a5\u9053\u9499\u548c\u6c34\u6bcd\u7d20\u53d1\u5149\u7684\u5173\u7cfb\u30021967\u5e74Ridgway\u548cAshley\u63d0\u51fa\u68c0\u6d4b\u9499\u7684 \u65b0\u65b9\u6cd5\uff1a\u7528\u6c34\u6bcd\u7d20\u3002\u9499\u79bb\u5b50\u662f\u751f\u7269\u4f53\u5185\u7684\u91cd\u8981\u4fe1\u53f7\u5206\u5b50\uff0c\u6c34\u6bcd\u7d20\u6210\u4e3a\u7b2c\u4e00\u4e2a\u6709\u7a7a\u95f4\u5206\u8fa8\u80fd\u529b\u7684\u9499\u68c0\u6d4b\u65b9\u6cd5\uff0c\u662f\u76ee\u524d\u4ecd\u7528\u7684\u65b9\u6cd5\u4e4b\u4e00\u3002<\/p>\n
1955 \u5e74 Davenport\u548cNicol\u53d1\u73b0\u6c34\u6bcd\u53ef\u4ee5\u53d1\u7eff\u5149\uff0c\u4f46\u4e0d\u77e5\u5176\u56e0\u30021962\u5e74\u4e0b\u6751\u4fee\u548c\u7ea6\u7ff0\u68ee\u90a3\u7bc7\u7eaf\u5316\u6c34\u6bcd\u7d20\u7684\u6587\u7ae0\u4e2d\uff0c\u6709\u4e2a\u6ce8\u811a\uff0c\u8bf4\u8fd8\u53d1\u73b0\u4e86\u53e6\u4e00\u79cd\u86cb\u767d\uff0c\u5b83 \u5728\u9633\u5149\u4e0b\u5448\u7eff\u8272\u3001\u94a8\u4e1d\u706f\u4e0b\u5448\u9ec4\u8272\u3001\u7d2b\u5916\u5149\u4e0b\u53d1\u5f3a\u70c8\u7eff\u8272\u3002\u5176\u540e\u4ed6\u4eec\u4ed4\u7ec6\u7814\u7a76\u4e86\u5176\u53d1\u5149\u7279\u6027\u30021974\u5e74\uff0c\u4ed6\u4eec\u5f97\u5230\u4e86\u8fd9\u4e2a\u86cb\u767d\uff0c\u5f53\u65f6\u79f0\u7eff\u8272\u86cb\u767d\u3001\u4ee5\u540e\u79f0\u7eff\u8272\u8367\u5149 \u86cb\u767d\uff08GFP\uff09\u3002Morin\u548cHastings\u63d0\u51fa\u6c34\u6bcd\u7d20\u548cGFP\u4e4b\u95f4\u53ef\u4ee5\u53d1\u751f\u80fd\u91cf\u8f6c\u79fb\u3002\u6c34\u6bcd\u7d20\u5728\u9499\u523a\u6fc0\u4e0b\u53d1\u5149\uff0c\u5176\u80fd\u91cf\u53ef\u8f6c\u79fb\u5230GFP\uff0c\u523a\u6fc0GFP\u53d1\u5149\u3002 \u8fd9\u662f\u7269\u7406\u5316\u5b66\u4e2d\u5df2\u77e5\u7684\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb(FRET)\u5728\u751f\u7269\u4e2d\u7684\u53d1\u73b0\u3002<\/p>\n
\u4e0b\u6751\u4fee\u672c\u4eba\u5bf9GFP\u7684\u5e94\u7528\u524d\u666f\u4e0d\u654f\u611f\uff0c\u4e5f\u672a\u610f\u8bc6\u5230\u5e94\u7528\u7684\u91cd\u8981\u6027\u3002 \u4ed6\u79bb\u5f00 \u666e\u6797\u65af\u987f\u5230Woods Hole\u6d77\u6d0b\u7814\u7a76\u6240\u540e\uff0c\u4ed6\u7684\u540c\u4e8b\u666e\u745e\u820d(Douglas Prasher)\u975e\u5e38\u611f\u5174\u8da3\u7528\u8367\u5149\u86cb\u767d\u505a\u751f\u7269\u793a\u8e2a\u5206\u5b50\u30021985\u5e74\u666e\u745e\u820d\u548c\u65e5\u88d4\u79d1\u5b66\u5bb6Satoshi Inouye\u5206\u522b\u6839\u636e\u86cb\u767d\u8d28\u5e8f\u5217\u62ff\u5230\u4e86\u6c34\u6bcd\u7d20\u7684\u57fa\u56e0\uff08\u751f\u7269\u5b66\u4e0a\u51c6\u786e\u5730\u8bf4\u662fcDNA\uff09\u30021992\u5e74\uff0c\u666e\u745e\u820d\u53c8\u62ff\u5230GFP\u7684\u57fa\u56e0\u3002\u6709\u4e86cDNA\uff0c\u4e00\u822c\u751f\u7269\u5b66\u7814 \u7a76\u8005\u5c31\u5f88\u5bb9\u6613\u5e94\u7528\uff0c\u6bd4\u7528\u86cb\u767d\u8d28\u65b9\u4fbf\u591a\u4e86\u3002<\/p>\n
\u666e\u745e\u820d1992\u5e74\u53d1\u8868GFP\u57fa\u56e0\u7684\u6587\u7ae0\u540e\uff0c\u79bb\u5f00\u79d1\u5b66\u754c\u3002\u539f\u56e0\u662f\u4ed6\u7533\u8bf7\u7f8e\u56fd\u56fd\u5bb6\u79d1\u5b66\u57fa\u91d1\u65f6\uff0c\u8bc4\u5ba1\u8005\u8bf4 \u6ca1\u6709\u86cb\u767d\u8d28\u53d1\u5149\u7684\u5148\u4f8b\uff0c\u5c31\u662f\u4ed6\u627e\u5230\u4e86\u8fd9\u79cd\u86cb\u767d\uff0c\u4e5f\u6ca1\u4ec0\u4e48\u4ef7\u503c\u3002\u4e00\u6c14\u4e4b\u4e0b\uff0c\u4ed6\u79bb\u5f00\u5b66\u672f\u754c\u53bb\u9ebb\u7701\u7a7a\u519b<\/u>\u56fd\u6c11\u536b\u961f\u57fa\u5730\uff0c\u5230\u519c\u4e1a\u90e8\u52a8\u690d\u7269\u670d\u52a1\u90e8\u5de5\u4f5c\u3002\u5f53\u65f6\u4ed6\u5982\u679c\u82b1\u51e0 \u7f8e\u5143\uff0c\u5c31\u53ef\u4ee5\u505a\u4e00\u4e2a\u4e00\u822c\u7814\u7a76\u751f\u90fd\u80fd\u505a\uff0c\u4f46\u975e\u5e38\u6f02\u4eae\u7684\u5de5\u4f5c\uff1a\u5c06\u6765\u81ea\u6c34\u6bcd\u7684GFP\u57fa\u56e0\u653e\u5230\u5176\u4ed6\u751f\u7269\u4f53\u5185\uff08\u5982\u7ec6\u83cc\uff09\uff0c\u770b\u5230\u8367\u5149\uff0c\u53ef\u4ee5\u5f88\u5f3a\u70c8\u5730\u63d0\u793aGFP\u672c\u8eab\u53ef\u4ee5 \u53d1\u5149\uff0c\u65e0\u9700\u5176\u4ed6\u5e95\u7269\u3001\u6216\u8005\u8f85\u52a9\u5206\u5b50\uff0c\u4e5f\u8868\u660e\u53ef\u4ee5\u5e7f\u6cdb\u7528GFP\u3002<\/p>\n
\u5c06GFP\u8868\u8fbe\u5230\u5176\u4ed6\u751f\u7269\u4f53\u8fd9\u9879\u5de5\u4f5c\uff0c1994\u5e74\u7531\u4e24\u4e2a\u5b9e\u9a8c\u5ba4\u72ec\u7acb\u8fdb\u884c\uff1a\u7f8e\u56fd\u54e5\u4f26\u6bd4\u4e9a\u5927\u5b66\u505a\u7ebf\u866b\u7684Marty Chalfie\u5b9e\u9a8c\u5ba4\uff0c\u548c\u52a0\u5dde\u5927\u5b66\u5723\u8fed\u54e5\u5206\u6821\u3001Scripps\u6d77\u6d0b\u7814\u7a76\u6240\u7684\u4e24\u4f4d\u65e5\u88d4\u79d1\u5b66\u5bb6Inouye\u548cTsuji\u3002<\/p>\n
\u6c34\u6bcd\u7d20\u548cGFP\u90fd\u6709\u91cd\u8981\u7684\u5e94\u7528\u3002\u4f46\u6c34\u6bcd\u7d20\u4ecd\u662f\u8367\u5149\u9176\u7684\u4e00\u79cd\uff0c\u5b83\u9700\u8367\u5149\u7d20\u3002\u800cGFP\u662f\u86cb\u767d\u8d28\u672c\u8eab\u53d1\u5149\uff0c\u539f\u7406\u4e0a\u4e0d\u540c\u3002<\/p>\n
Chalfie\u7684\u6587\u7ae0\u7acb\u5373\u5f15\u8d77\u8f70\u52a8\uff0c\u5f88\u591a\u751f\u7269\u5b66\u7814\u7a76\u8005\u63a5\u7740\u7eb7\u7eb7\u5c06GFP\u5f15\u5165\u81ea\u5df1\u7684\u7cfb\u7edf\u3002\u5f53\u65f6\u597d\u4e9b\u300a\u81ea\u7136\u300b\u3001\u300a\u79d1\u5b66\u300b\u6587\u7ae0\uff0c\u8bc1\u660e\u54ea\u91cc\u8868\u8fbeGFP\uff0c\u54ea\u91cc\u5c31\u6709\u7eff\u5149\uff0c\u8fd9\u4e9b\u540e\u7eed\u6587\u7ae0\u4e0d\u8fc7\u662f\u8ddf\u98ce\uff0c\u4e0a\u4e86\u300a\u81ea\u7136\u300b\u4e5f\u4e0d\u8bc1\u660e\u6709\u539f\u521b\u6027\u3002<\/p>\n
1994 \u5e74\uff0c\u534e\u88d4\u7f8e\u56fd\u79d1\u5b66\u5bb6\u94b1\u6c38\u5065\uff08Roger Y Tsien\uff09\u5f00\u59cb\u6539\u9020GFP\uff0c\u6709\u591a\u9879\u53d1\u73b0\u3002\u4e16\u754c\u4e0a\u76ee\u524d\u4f7f\u7528\u7684\u8367\u5149\u86cb\u767d\u5927\u591a\u662f\u94b1\u6c38\u5065\u5b9e\u9a8c\u5ba4\u6539\u9020\u540e\u7684\u53d8\u79cd\uff0c\u6709\u7684\u8367\u5149\u66f4\u5f3a\uff0c\u6709\u7684\u5448\u9ec4\u8272\u3001\u84dd\u8272\uff0c\u6709\u7684\u53ef\u6fc0\u6d3b\u3001\u53ef\u53d8 \u8272\u3002\u7528\u4e00\u4e9b\u4e0d\u5e38\u7528\u505a\u7814\u7a76\u6837\u672c\u7684\u751f\u7269\u627e\u6709\u989c\u8272\u7684\u86cb\u767d\u6210\u4e3a\u4e00\u4e9b\u4eba\u7684\u7231\u597d\u3002\u4e0d\u8fc7\u771f\u53d1\u73b0\u7684\u6709\u7528\u4e1c\u897f\u5e76\u4e0d\u5f88\u591a\u3002\u6210\u529f\u7684\u4f8b\u5b50\u6709\u4fc4\u56fd\u79d1\u5b66\u9662\u751f\u7269\u6709\u673a\u5316\u5b66\u7814\u7a76\u6240 Sergey A. Lukyanov\u5b9e\u9a8c\u5ba4\u4ece\u73ca\u745a\u91cc\u53d1\u73b0\u7684\u5176\u4ed6\u8367\u5149\u86cb\u767d\uff08FP\uff09\uff0c\u5305\u62ec\u7ea2\u8272\u8367\u5149\u86cb\u767d\u3002<\/p>\n
\u7efc\u89c2\u6574\u4e2a\u8fc7\u7a0b\uff0c\u4ece1961\u5e74\u5230 1974\u5e74\uff0c\u4e0b\u6751\u4fee\u548c\u7ea6\u7ff0\u68ee\u7684\u7814\u7a76\u9065\u9065\u9886\u5148\uff0c\u4f46\u5f88\u5c11\u4eba\u6ce8\u610f\u3002\u5355\u7eaf\u4ece\u6280\u672f\u4e0a\uff0c\u5176\u4ed6\u751f\u5316\u5b66\u5bb6\u4e5f\u53ef\u4ee5\u5f97\u5230\u6c34\u6bcd\u7d20\u548cGFP\uff0c\u4f46\u9700\u8981\u6709\u60f3\u6cd5\u6216\u5174\u8da3\u3002\u57281974\u5e74\u4ee5 \u540e\uff0c\u7279\u522b\u662f\u516b\u5341\u5e74\u4ee3\u540e\uff0c\u5f88\u591a\u540e\u7eed\u5de5\u4f5c\u663e\u800c\u6613\u89c1\uff0c\u4e00\u822c\u7814\u7a76\u751f\u53ef\u4ee5\u505a\u3002\u5176\u4e2d\u4f8b\u5916\u662f\u94b1\u6c38\u5065\u5b9e\u9a8c\u5ba4\u53d1\u73b0\u53d8\u79cd\u51fa\u73b0\u65b0\u989c\u8272\uff0c\u8fd9\u4e00\u53d1\u73b0\u51fa\u4e4e\u610f\u6599\u3002<\/p>\n
GFP\u4e4b\u7f8e\u4e3d\u548c\u5999\u7528<\/p>\n
GFP\u53ca\u5176\u884d\u751f\u7269\uff08\u5404\u79cd\u8367\u5149\u86cb\u767d\uff09\uff0c\u7eda\u4e3d\u591a\u5f69\uff0c\u975e\u5e38\u6f02\u4eae\u3002<\/p>\n
\u6709\u4e9b\u8367\u5149\u86cb\u767d\u5f53\u6d53\u5ea6\u8db3\u591f\u9ad8\u65f6\uff0c\u5728\u65e5\u5149\u4e0b\u53ef\u4ee5\u770b\u5230\u989c\u8272\u3002\u6240\u4ee5\u5b9e\u9a8c\u5ba4\u4ea7\u751f\u4e86\u4eba\u4e3a\u53ef\u63a7\u5236\u989c\u8272\u7684\u9c7c\u3001\u8001\u9f20\u3002<\/p>\n
\u8367 \u5149\u86cb\u767d\u5e7f\u6cdb\u5e94\u7528\u4e8e\u751f\u7269\u5b66\u7814\u7a76\u3002\u53ef\u4ee5\u901a\u8fc7\u5e38\u89c4\u7684\u57fa\u56e0\u64cd\u7eb5\u624b\u6bb5\uff0c\u5c06\u8367\u5149\u86cb\u767d\u7528\u6765\u6807\u8bb0\u5176\u4ed6\u76ee\u6807\u86cb\u767d\uff0c\u8fd9\u6837\u53ef\u4ee5\u89c2\u5bdf\u3001\u8ddf\u8e2a\u76ee\u6807\u86cb\u767d\u7684\u65f6\u95f4\u3001\u7a7a\u95f4\u53d8\u5316\u3002\u63d0\u4f9b\u4e86\u4ee5\u524d\u4e0d \u80fd\u8fbe\u5230\u7684\u65f6\u95f4\u548c\u7a7a\u95f4\u5206\u8fa8\u7387\uff0c\u800c\u4e14\u53ef\u4ee5\u5728\u6d3b\u7ec6\u80de\u3001\u751a\u81f3\u6d3b\u4f53\u52a8\u7269\u4e2d\u89c2\u5bdf\u5230\u4e00\u4e9b\u5206\u5b50\u3002\u8367\u5149\u86cb\u767d\u6280\u672f\u4e5f\u4f7f\u5f97\u4eba\u4eec\u53ef\u4ee5\u7814\u7a76\u67d0\u4e9b\u5206\u5b50\u7684\u6d3b\u6027\uff0c\u800c\u4e0d\u4ec5\u4ec5\u662f\u5176\u5b58\u5728\u4e0e\u5426\u3002<\/p>\n
\u5bf9\u4e8e\u6709\u4e9b\u7814\u7a76\u6765\u8bf4\uff0c\u8367\u5149\u86cb\u767d\u7684\u4f5c\u7528\u53ef\u4ee5\u5f62\u5bb9\u4e3a\u201c\u8d77\u6b7b\u56de\u751f\u201d\uff1a\u539f\u6765\u6709\u4e9b\u65b9\u6cd5\uff0c\u9700\u8981\u628a\u751f\u7269\u53d8\u6210\u6b7b\u7269\u624d\u80fd\u7814\u7a76\u4e00\u4e9b\u73b0\u8c61\u548c\u8fc7\u7a0b\uff0c\u800c\u8367\u5149\u86cb\u767d\u4e3a\u4e3b\u8981\u652f\u67f1\u4e4b\u4e00\u7684\u73b0\u4ee3\u6210\u50cf\u6280\u672f\uff0c\u4f7f\u79d1\u5b66\u5bb6\u5728\u6d3b\u7684\u7ec6\u80de\u4e2d\u89c2\u5bdf\u548c\u7814\u7a76\u8fd9\u4e9b\u8fc7\u7a0b\uff0c\u4f7f\u4e00\u90e8\u5206\u201c\u6b7b\u7269\u5b66\u201d\u53d8\u6210\u201c\u751f\u7269\u5b66\u201d\u3002<\/p>\n
\u4e3a\u4e86\u597d\u5947<\/p>\n
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\u4e0b\u6751\u4fee\u52a0\u5165\u751f\u7269\u53d1\u5149\u7814\u7a76\u662f1955\u5e74\u5728\u65e5\u672c\u505a\u7814\u7a76\u751f\u65f6\uff0c\u5bfc \u5e08\u8ba9\u4ed6\u5230\u53e6\u5916\u4e00\u4e2a\u5b9e\u9a8c\u5ba4\u53bb\u5f00\u9614\u773c\u754c\uff0c\u800c\u90a3\u4e2a\u5b9e\u9a8c\u5ba4\u7684\u5bfc\u5e08\u4ecb\u7ecd\u4ed6\u505a\u8367\u5149\u7d20\u30021959\u5e74\u5bfc\u5e08\u901d\u4e8e\u764c\u75c7<\/u>\uff0c1960 \u5e74\u4ed6\u5230\u7ea6\u7ff0\u68ee\u5b9e\u9a8c\u5ba4\u3002\u7ea6\u7ff0\u68ee\u7ed9\u4ed6\u770b\u6c34\u6bcd\u53d1\u5149\uff0c\u8981 \u4ed6\u505a\uff0c\u53ef\u662f\u7b2c\u4e00\u6b21\u6f14\u793a\u6839\u672c\u6ca1\u6709\u53d1\u5149\u3002\u4f46\u4e0b\u6751\u4fee\u88ab\u7ea6\u7ff0\u68ee\u611f\u67d3\u4e86\uff0c\u51b3\u5b9a\u505a\u8fd9\u4e2a\u8bfe\u9898\u30021961\u5e74\u7ea6\u7ff0\u68ee\u5f00\u4e86\u4e03\u5929\u7684\u8f66\u3001\u6bcf\u592912\u5c0f\u65f6\uff0c\u5e26\u4e0b\u6751\u4fee\u6a2a\u8de8\u7f8e\u56fd\u5230\u897f\u6d77\u5cb8\u534e \u76db\u987f\u5dde\u7684\u201c\u661f\u671f\u4e94\u6e2f\u201d\uff08Friday Harbor\uff09\u5b9e\u9a8c\u5ba4\uff0c\u90a3\u91cc\u5f53\u65f6\u76db\u4ea7\u6c34\u6bcd\uff0c\u6709\u5f88\u591a\u539f\u6599\uff0c\u4ed6\u4eec\u57281961\u5e74\u590f\u505a\u51fa\u4e3b\u8981\u53d1\u73b0\u3002<\/p>\n
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